GPR4 - Gs

GPCR CLASS (FAMILY)

Class A (Rhodopsin)

GPCR SPECIES

Homo sapiens

GPCR PREFERRED CHAIN

R

G PROTEIN (FAMILY)

Gs

G PROTEIN SPECIES

Homo sapiens

PDB CODE

8ZCF

RESOLUTION

2.9 Å

REFERENCE

10.1038/S41422-025-01092-W

PUBLICATION DATE

April 23, 2025

3D VIEW

NGL is a WebGL based 3D viewer powered by MMTF.

LIGAND INTERACTIONS

0 interactions (Click to see)

LIGANDS

Apo (no ligand) -

PHYSIOLOGICAL LIGANDS

No physiological ligands available



GPCR - G protein interface interactions



The biflare plot is a modified version of a flareplot, where lines are drawn between an inner concentric circle (Gα residues) and an outer concentric circle (GPCR residues) depicting the interface interactions.
Different interaction types are shown with different line types (aromatic - solid, hydrophobic - dashed, ionic - long dashed, polar - dotted, Van der Waals - dash-dot).
The interactive features include filtering on interaction type, segments, residues and backbone or sidechain interactions.


Interaction definitions:

Residue numbering:

GPCR-G protein backbone (bb) or sidechain (sc):





Structures

Sequence Color

Gα, Gβ, Gγ, GPCR
Interaction cut-off: strict (stick), loose (line)

NGL is a WebGL based 3D viewer powered by MMTF.

Interaction
type		 GPCR
Segment
AA
Number		 Generic
number		 Gα
segment
AA
Number		 Generic
number
Polar TM3 A 120 3x55 G.H5 R 380 G.H5.12
Van-der-waals TM3 A 120 3x55 G.H5 R 380 G.H5.12
Hydrophobic ICL3 S 213 - G.h4s6 Y 358 G.h4s6.20
Polar ICL3 T 214 - G.H5 R 385 G.H5.17
Hydrophobic ICL3 T 214 - G.H5 R 385 G.H5.17
Van-der-waals ICL3 T 214 - G.H5 R 385 G.H5.17
Polar TM6 K 221 6x32 G.H5 L 394 G.H5.26
Hydrophobic TM6 K 221 6x32 G.H5 L 394 G.H5.26
Van-der-waals TM6 K 221 6x32 G.H5 L 394 G.H5.26
Hydrophobic ICL2 L 123 - G.S1 H 41 G.S1.02
Van-der-waals ICL2 L 123 - G.S1 H 41 G.S1.02
Hydrophobic TM3 A 118 3x53 G.H5 Y 391 G.H5.23
Van-der-waals TM3 A 118 3x53 G.H5 Y 391 G.H5.23
Polar TM1 Q 45 1x60 G.H5 E 392 G.H5.24
Hydrophobic TM6 I 222 6x33 G.H5 L 394 G.H5.26
Van-der-waals TM6 I 222 6x33 G.H5 L 394 G.H5.26
Hydrophobic ICL2 L 123 - G.H5 R 380 G.H5.12
Polar H8 N 290 8x47 G.H5 E 392 G.H5.24
Hydrophobic H8 N 290 8x47 G.H5 E 392 G.H5.24
Van-der-waals H8 N 290 8x47 G.H5 E 392 G.H5.24
Hydrophobic TM6 I 222 6x33 G.H5 L 393 G.H5.25
Van-der-waals TM6 I 222 6x33 G.H5 L 393 G.H5.25
Hydrophobic TM3 R 115 3x50 G.H5 L 393 G.H5.25
Polar ICL2 R 129 - G.H5 Y 391 G.H5.23
Hydrophobic ICL2 R 129 - G.H5 Y 391 G.H5.23
Van-der-waals ICL2 R 129 - G.H5 Y 391 G.H5.23
Polar ICL2 P 122 - G.H5 H 387 G.H5.19
Hydrophobic ICL2 P 122 - G.H5 H 387 G.H5.19
Van-der-waals ICL2 P 122 - G.H5 H 387 G.H5.19
Hydrophobic TM3 V 119 3x54 G.H5 L 393 G.H5.25
Van-der-waals TM3 V 119 3x54 G.H5 L 393 G.H5.25
Hydrophobic ICL2 R 129 - G.H5 H 387 G.H5.19
Ionic TM6 E 218 6x29 G.H5 R 385 G.H5.17
Polar TM6 E 218 6x29 G.H5 R 385 G.H5.17
Hydrophobic TM6 E 218 6x29 G.H5 R 385 G.H5.17
Van-der-waals TM6 E 218 6x29 G.H5 R 385 G.H5.17
Hydrophobic ICL2 P 122 - G.H5 Q 384 G.H5.16
Hydrophobic TM6 E 218 6x29 G.H5 L 394 G.H5.26
Van-der-waals TM6 E 218 6x29 G.H5 L 394 G.H5.26
Polar TM2 N 50 2x37 G.H5 Q 390 G.H5.22
Van-der-waals TM2 N 50 2x37 G.H5 Q 390 G.H5.22
Hydrophobic TM6 L 225 6x36 G.H5 L 393 G.H5.25
Hydrophobic TM3 V 119 3x54 G.H5 L 388 G.H5.20
Van-der-waals TM3 V 119 3x54 G.H5 L 388 G.H5.20
Hydrophobic ICL2 L 123 - G.S3 V 217 G.S3.01
Hydrophobic TM5 A 207 5x64 G.H5 Q 384 G.H5.16
Van-der-waals TM5 A 207 5x64 G.H5 Q 384 G.H5.16
Polar ICL3 V 212 - G.h4s6 Y 358 G.h4s6.20
Hydrophobic ICL3 V 212 - G.h4s6 Y 358 G.h4s6.20
Polar ICL3 S 213 - G.H5 R 385 G.H5.17
Hydrophobic ICL3 S 213 - G.H5 R 385 G.H5.17
Van-der-waals ICL3 S 213 - G.H5 R 385 G.H5.17
Polar TM3 V 119 3x54 G.H5 Q 384 G.H5.16
Hydrophobic TM3 V 119 3x54 G.H5 Q 384 G.H5.16
Van-der-waals TM3 V 119 3x54 G.H5 Q 384 G.H5.16
Hydrophobic H8 A 293 8x50 G.H5 E 392 G.H5.24
Polar TM3 D 114 3x49 G.H5 Y 391 G.H5.23
Hydrophobic TM3 D 114 3x49 G.H5 Y 391 G.H5.23
Van-der-waals TM3 D 114 3x49 G.H5 Y 391 G.H5.23
Hydrophobic ICL2 P 122 - G.H5 I 383 G.H5.15
Van-der-waals ICL2 P 122 - G.H5 I 383 G.H5.15
Hydrophobic TM5 I 204 5x61 G.H5 L 393 G.H5.25
Van-der-waals TM5 I 204 5x61 G.H5 L 393 G.H5.25
Van-der-waals TM7 Y 286 7x53 G.H5 E 392 G.H5.24
Polar TM3 R 115 3x50 G.H5 Y 391 G.H5.23
Hydrophobic TM3 R 115 3x50 G.H5 Y 391 G.H5.23
Van-der-waals TM3 R 115 3x50 G.H5 Y 391 G.H5.23
Hydrophobic TM3 V 119 3x54 G.H5 H 387 G.H5.19
Hydrophobic ICL2 L 123 - G.H5 I 383 G.H5.15
Van-der-waals ICL2 L 123 - G.H5 I 383 G.H5.15
Ionic TM3 R 115 3x50 G.H5 E 392 G.H5.24
Polar TM3 R 115 3x50 G.H5 E 392 G.H5.24
Hydrophobic ICL2 P 122 - G.H5 R 380 G.H5.12
Hydrophobic TM5 V 208 5x65 G.H5 L 388 G.H5.20
Polar ICL3 S 213 - G.H5 D 381 G.H5.13
Hydrophobic ICL3 S 213 - G.H5 D 381 G.H5.13
Van-der-waals ICL3 S 213 - G.H5 D 381 G.H5.13
Hydrophobic ICL3 S 213 - G.S6 Y 360 G.S6.02
Hydrophobic ICL2 L 123 - G.H5 F 376 G.H5.08
Van-der-waals ICL2 L 123 - G.H5 F 376 G.H5.08
Polar TM5 S 211 5x68 G.H5 Q 384 G.H5.16
Hydrophobic TM5 S 211 5x68 G.H5 Q 384 G.H5.16
Van-der-waals TM5 S 211 5x68 G.H5 Q 384 G.H5.16
Hydrophobic TM2 L 52 2x39 G.H5 Y 391 G.H5.23
Polar ICL3 V 212 - G.H5 D 381 G.H5.13
Hydrophobic ICL3 V 212 - G.H5 D 381 G.H5.13
Van-der-waals ICL3 V 212 - G.H5 D 381 G.H5.13
Hydrophobic TM6 I 222 6x33 G.H5 L 388 G.H5.20
Van-der-waals TM6 I 222 6x33 G.H5 L 388 G.H5.20
Polar TM3 A 118 3x53 G.H5 H 387 G.H5.19
Hydrophobic TM3 A 118 3x53 G.H5 H 387 G.H5.19
Van-der-waals TM3 A 118 3x53 G.H5 H 387 G.H5.19