Gs - GPR32 complex model

GPCR CLASS (FAMILY)

A (Rhodopsin)

SPECIES

Homo sapiens

G PROTEIN FAMILY

Gs

DATE

2023-09-15

3D VIEW

NGL is a WebGL based 3D viewer powered by MMTF.


Tertiary structure confidence

Chain pLDDT score avg
GPR32 77.8
Gαs 86.0
Very high (>90)
Confident (90-70)
Low (70-50)
Very low (<50)
Disordered

PTM Score: 0.7
iPTM Score: 0.6
PAE mean: 15.2


GPCR - G protein interface interactions



The biflare plot is a modified version of a flareplot, where lines are drawn between an inner concentric circle (Gα residues) and an outer concentric circle (GPCR residues) depicting the interface interactions.
Different interaction types are shown with different line types (aromatic - solid, hydrophobic - dashed, ionic - long dashed, polar - dotted, Van der Waals - dash-dot).
The interactive features include filtering on interaction type, segments, residues and backbone or sidechain interactions.


Interaction definitions:

Residue numbering:

GPCR-G protein backbone (bb) or sidechain (sc):





Structures

Sequence Color

Gα, GPCR
Interaction cut-off: strict (stick), loose (line)

NGL is a WebGL based 3D viewer powered by MMTF.

Interaction
type		 GPCR
Segment
AA
Number		 Generic
number		 Gα
segment
AA
Number		 Generic
number
Polar ICL2 R 153 34x57 G.H5 Q 390 G.H5.22
Van-der-waals ICL2 R 153 34x57 G.H5 Q 390 G.H5.22
Polar TM3 S 142 3x53 G.H5 Y 391 G.H5.23
Hydrophobic TM3 S 142 3x53 G.H5 Y 391 G.H5.23
Polar TM3 R 139 3x50 G.H5 Y 391 G.H5.23
Hydrophobic TM3 R 139 3x50 G.H5 Y 391 G.H5.23
Van-der-waals TM3 R 139 3x50 G.H5 Y 391 G.H5.23
Hydrophobic TM5 L 243 5x65 G.H5 L 388 G.H5.20
Polar TM6 P 254 6x33 G.H5 L 393 G.H5.25
Hydrophobic TM6 P 254 6x33 G.H5 L 393 G.H5.25
Van-der-waals TM6 P 254 6x33 G.H5 L 393 G.H5.25
Polar H8 R 321 8x48 G.H5 E 392 G.H5.24
Hydrophobic ICL2 P 146 34x50 G.H5 H 387 G.H5.19
Van-der-waals ICL2 P 146 34x50 G.H5 H 387 G.H5.19
Hydrophobic TM2 S 77 2x39 G.H5 Y 391 G.H5.23
Polar TM3 D 138 3x49 G.H5 Y 391 G.H5.23
Hydrophobic TM3 D 138 3x49 G.H5 Y 391 G.H5.23
Van-der-waals TM3 D 138 3x49 G.H5 Y 391 G.H5.23
Polar TM5 E 246 5x68 G.H5 Q 384 G.H5.16
Hydrophobic TM5 E 246 5x68 G.H5 Q 384 G.H5.16
Van-der-waals TM5 E 246 5x68 G.H5 Q 384 G.H5.16
Hydrophobic TM6 L 258 6x37 G.H5 L 393 G.H5.25
Hydrophobic ICL2 L 150 34x54 G.H5 M 386 G.H5.18
Hydrophobic ICL3 W 248 - G.H5 L 394 G.H5.26
Van-der-waals ICL3 W 248 - G.H5 L 394 G.H5.26
Hydrophobic ICL2 P 146 34x50 G.H5 I 383 G.H5.15
Hydrophobic ICL2 N 151 34x55 G.hns1 A 39 G.hns1.03
Van-der-waals ICL2 N 151 34x55 G.hns1 A 39 G.hns1.03
Polar TM6 R 253 6x32 G.H5 L 394 G.H5.26
Hydrophobic TM6 R 253 6x32 G.H5 L 394 G.H5.26
Van-der-waals TM6 R 253 6x32 G.H5 L 394 G.H5.26
Hydrophobic TM6 L 257 6x36 G.H5 L 393 G.H5.25
Polar ICL3 W 248 - G.H5 D 381 G.H5.13
Hydrophobic ICL3 W 248 - G.H5 D 381 G.H5.13
Van-der-waals ICL3 W 248 - G.H5 D 381 G.H5.13
Hydrophobic ICL2 P 146 34x50 G.H5 R 380 G.H5.12
Van-der-waals ICL2 P 146 34x50 G.H5 R 380 G.H5.12
Polar TM3 V 143 3x54 G.H5 Q 384 G.H5.16
Hydrophobic TM3 V 143 3x54 G.H5 Q 384 G.H5.16
Van-der-waals TM3 V 143 3x54 G.H5 Q 384 G.H5.16
Polar TM2 S 77 2x39 G.H5 Q 390 G.H5.22
Hydrophobic TM2 S 77 2x39 G.H5 Q 390 G.H5.22
Van-der-waals TM2 S 77 2x39 G.H5 Q 390 G.H5.22
Hydrophobic ICL2 L 150 34x54 G.S1 H 41 G.S1.02
Van-der-waals ICL2 L 150 34x54 G.S1 H 41 G.S1.02
Aromatic ICL3 W 248 - G.H5 R 385 G.H5.17
Hydrophobic ICL3 W 248 - G.H5 R 385 G.H5.17
Van-der-waals ICL3 W 248 - G.H5 R 385 G.H5.17
Hydrophobic TM3 V 143 3x54 G.H5 L 388 G.H5.20
Van-der-waals TM3 V 143 3x54 G.H5 L 388 G.H5.20
Ionic H8 D 322 8x49 G.H5 R 389 G.H5.21
Polar H8 D 322 8x49 G.H5 R 389 G.H5.21
Hydrophobic ICL2 L 150 34x54 G.H5 I 383 G.H5.15
Polar H8 D 322 8x49 G.H5 E 392 G.H5.24
Van-der-waals TM7 V 317 7x54 G.H5 E 392 G.H5.24
Hydrophobic TM3 R 139 3x50 G.H5 L 393 G.H5.25
Hydrophobic ICL2 A 149 34x53 G.H5 H 387 G.H5.19
Van-der-waals ICL2 A 149 34x53 G.H5 H 387 G.H5.19
Hydrophobic TM5 K 242 5x64 G.H5 Q 384 G.H5.16
Polar TM7 V 319 7x56 G.H5 E 392 G.H5.24
Hydrophobic ICL2 V 147 34x51 G.S3 V 217 G.S3.01
Van-der-waals ICL2 V 147 34x51 G.S3 V 217 G.S3.01
Ionic TM5 E 246 5x68 G.H5 R 380 G.H5.12
Polar TM5 E 246 5x68 G.H5 R 380 G.H5.12
Van-der-waals TM5 E 246 5x68 G.H5 R 380 G.H5.12
Hydrophobic ICL2 L 150 34x54 G.H5 H 387 G.H5.19
Polar H8 F 323 8x50 G.H5 E 392 G.H5.24
Hydrophobic H8 F 323 8x50 G.H5 E 392 G.H5.24
Van-der-waals H8 F 323 8x50 G.H5 E 392 G.H5.24
Polar TM4 Q 156 4x40 G.HN Q 31 G.HN.48
Van-der-waals TM4 Q 156 4x40 G.HN Q 31 G.HN.48
Polar ICL2 R 153 34x57 G.H5 Y 391 G.H5.23
Hydrophobic ICL2 R 153 34x57 G.H5 Y 391 G.H5.23
Van-der-waals ICL2 R 153 34x57 G.H5 Y 391 G.H5.23
Van-der-waals ICL2 L 150 34x54 G.hns1 R 38 G.hns1.02
Polar TM3 S 142 3x53 G.H5 H 387 G.H5.19
Hydrophobic TM3 S 142 3x53 G.H5 H 387 G.H5.19
Van-der-waals TM3 S 142 3x53 G.H5 H 387 G.H5.19
Hydrophobic ICL3 W 248 - G.H5 L 388 G.H5.20
Van-der-waals ICL3 W 248 - G.H5 L 388 G.H5.20
Ionic H8 E 325 8x52 G.h4s6 R 356 G.h4s6.12
Polar H8 E 325 8x52 G.h4s6 R 356 G.h4s6.12
Van-der-waals H8 E 325 8x52 G.h4s6 R 356 G.h4s6.12
Hydrophobic ICL2 P 146 34x50 G.H5 Q 384 G.H5.16
Polar H8 G 320 8x47 G.H5 E 392 G.H5.24
Hydrophobic H8 G 320 8x47 G.H5 E 392 G.H5.24
Van-der-waals H8 G 320 8x47 G.H5 E 392 G.H5.24