G12 - P2RY8 complex model

GPCR CLASS (FAMILY)

A (Rhodopsin)

SPECIES

Homo sapiens

G PROTEIN FAMILY

G12/13

DATE

2023-09-15

3D VIEW

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Tertiary structure confidence

Chain pLDDT score avg
P2RY8 77.3
subunit alpha-12 83.3
Very high (>90)
Confident (90-70)
Low (70-50)
Very low (<50)
Disordered

PTM Score: 0.7
iPTM Score: 0.7
PAE mean: 15.5


GPCR - G protein interface interactions



The biflare plot is a modified version of a flareplot, where lines are drawn between an inner concentric circle (Gα residues) and an outer concentric circle (GPCR residues) depicting the interface interactions.
Different interaction types are shown with different line types (aromatic - solid, hydrophobic - dashed, ionic - long dashed, polar - dotted, Van der Waals - dash-dot).
The interactive features include filtering on interaction type, segments, residues and backbone or sidechain interactions.


Interaction definitions:

Residue numbering:

GPCR-G protein backbone (bb) or sidechain (sc):





Structures

Sequence Color

Gα, GPCR
Interaction cut-off: strict (stick), loose (line)

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Interaction
type		 GPCR
Segment
AA
Number		 Generic
number		 Gα
segment
AA
Number		 Generic
number
Hydrophobic TM6 L 238 6x37 G.H5 M 379 G.H5.24
Hydrophobic TM7 A 296 7x56 G.H5 M 379 G.H5.24
Van-der-waals TM7 A 296 7x56 G.H5 M 379 G.H5.24
Hydrophobic C-term R 322 - G.H3 K 284 G.H3.16
Van-der-waals C-term R 322 - G.H3 K 284 G.H3.16
Polar C-term R 354 - H.HA D 113 H.HA.27
Polar TM3 R 121 3x50 G.H5 I 378 G.H5.23
Hydrophobic TM3 R 121 3x50 G.H5 I 378 G.H5.23
Van-der-waals TM3 R 121 3x50 G.H5 I 378 G.H5.23
Polar ICL2 R 135 34x57 G.H5 N 374 G.H5.19
Hydrophobic ICL2 R 135 34x57 G.H5 N 374 G.H5.19
Van-der-waals ICL2 R 135 34x57 G.H5 N 374 G.H5.19
Hydrophobic ICL2 L 129 34x51 G.S3 I 221 G.S3.01
Van-der-waals ICL2 L 129 34x51 G.S3 I 221 G.S3.01
Hydrophobic ICL2 P 128 34x50 G.H5 L 371 G.H5.16
Polar TM6 Q 231 6x30 G.H5 L 375 G.H5.20
Hydrophobic TM6 Q 231 6x30 G.H5 L 375 G.H5.20
Van-der-waals TM6 Q 231 6x30 G.H5 L 375 G.H5.20
Hydrophobic ICL2 L 129 34x51 G.S1 V 58 G.S1.02
Van-der-waals ICL2 L 129 34x51 G.S1 V 58 G.S1.02
Hydrophobic TM6 R 232 6x31 G.H5 L 375 G.H5.20
Polar H8 S 297 8x47 G.H5 L 380 G.H5.25
Hydrophobic H8 S 297 8x47 G.H5 L 380 G.H5.25
Van-der-waals H8 S 297 8x47 G.H5 L 380 G.H5.25
Ionic C-term E 356 - G.s4h3 R 265 G.s4h3.12
Polar C-term E 356 - G.s4h3 R 265 G.s4h3.12
Hydrophobic C-term E 356 - G.s4h3 R 265 G.s4h3.12
Van-der-waals C-term E 356 - G.s4h3 R 265 G.s4h3.12
Hydrophobic C-term F 359 - G.s4h3 M 262 G.s4h3.09
Van-der-waals C-term F 359 - G.s4h3 M 262 G.s4h3.09
Hydrophobic TM3 V 125 3x54 G.H5 L 375 G.H5.20
Hydrophobic TM3 V 125 3x54 G.H5 I 378 G.H5.23
Van-der-waals TM3 V 125 3x54 G.H5 I 378 G.H5.23
Hydrophobic ICL3 E 225 - G.H5 D 368 G.H5.13
Van-der-waals ICL3 E 225 - G.H5 D 368 G.H5.13
Polar C-term S 357 - G.s4h3 R 265 G.s4h3.12
Van-der-waals C-term S 357 - G.s4h3 R 265 G.s4h3.12
Hydrophobic C-term F 359 - H.HA K 103 H.HA.17
Van-der-waals C-term F 359 - H.HA K 103 H.HA.17
Hydrophobic ICL2 R 133 34x55 G.hns1 R 56 G.hns1.03
Van-der-waals ICL2 R 133 34x55 G.hns1 R 56 G.hns1.03
Hydrophobic ICL2 L 129 34x51 G.H5 F 363 G.H5.08
Van-der-waals ICL2 L 129 34x51 G.H5 F 363 G.H5.08
Hydrophobic H8 R 298 8x48 G.H5 Q 381 G.H5.26
Polar TM3 G 124 3x53 G.H5 N 374 G.H5.19
Hydrophobic TM3 G 124 3x53 G.H5 N 374 G.H5.19
Van-der-waals TM3 G 124 3x53 G.H5 N 374 G.H5.19
Hydrophobic TM4 R 136 4x38 G.hns1 R 55 G.hns1.02
Van-der-waals TM4 R 136 4x38 G.hns1 R 55 G.hns1.02
Aromatic ICL3 H 227 - G.S6 F 347 G.S6.02
Hydrophobic ICL3 H 227 - G.S6 F 347 G.S6.02
Van-der-waals ICL3 H 227 - G.S6 F 347 G.S6.02
Polar ICL3 H 227 - G.H5 Q 372 G.H5.17
Van-der-waals ICL3 H 227 - G.H5 Q 372 G.H5.17
Hydrophobic TM3 V 125 3x54 G.H5 L 371 G.H5.16
Van-der-waals TM3 V 125 3x54 G.H5 L 371 G.H5.16
Hydrophobic TM7 Y 293 7x53 G.H5 L 380 G.H5.25
Hydrophobic TM6 A 235 6x34 G.H5 L 375 G.H5.20
Van-der-waals TM6 A 235 6x34 G.H5 L 375 G.H5.20
Polar H8 S 297 8x47 G.H5 M 379 G.H5.24
Hydrophobic H8 S 297 8x47 G.H5 M 379 G.H5.24
Van-der-waals H8 S 297 8x47 G.H5 M 379 G.H5.24
Hydrophobic ICL2 L 129 34x51 G.H5 V 366 G.H5.11
Polar C-term R 354 - H.HA K 114 H.HA.28
Hydrophobic C-term R 354 - H.HA K 114 H.HA.28
Van-der-waals C-term R 354 - H.HA K 114 H.HA.28
Hydrophobic ICL2 L 129 34x51 G.H5 K 367 G.H5.12
Hydrophobic TM2 V 59 2x40 G.H5 L 380 G.H5.25
Hydrophobic TM6 Q 231 6x30 G.H5 M 379 G.H5.24
Van-der-waals TM6 Q 231 6x30 G.H5 M 379 G.H5.24
Polar C-term Q 355 - H.HA K 114 H.HA.28
Van-der-waals C-term Q 355 - H.HA K 114 H.HA.28
Polar C-term D 319 - G.H3 K 284 G.H3.16
Van-der-waals C-term D 319 - G.H3 K 284 G.H3.16
Hydrophobic TM6 R 232 6x31 G.H5 L 371 G.H5.16
Van-der-waals TM6 R 232 6x31 G.H5 L 371 G.H5.16
Hydrophobic ICL2 P 128 34x50 G.H5 K 367 G.H5.12
Van-der-waals ICL2 P 128 34x50 G.H5 K 367 G.H5.12
Hydrophobic ICL1 M 52 - G.H5 L 380 G.H5.25
Van-der-waals ICL1 M 52 - G.H5 L 380 G.H5.25
Hydrophobic ICL2 P 128 34x50 G.H5 I 370 G.H5.15
Van-der-waals ICL2 P 128 34x50 G.H5 I 370 G.H5.15
Hydrophobic C-term F 359 - H.HA V 107 H.HA.21
Van-der-waals C-term F 359 - H.HA V 107 H.HA.21
Hydrophobic TM6 A 235 6x34 G.H5 M 379 G.H5.24
Polar C-term S 357 - H.HA D 110 H.HA.24
Hydrophobic C-term S 357 - H.HA D 110 H.HA.24
Van-der-waals C-term S 357 - H.HA D 110 H.HA.24
Ionic ICL3 E 225 - G.H5 H 364 G.H5.09
Polar ICL3 E 225 - G.H5 H 364 G.H5.09
Hydrophobic ICL3 E 225 - G.H5 H 364 G.H5.09
Van-der-waals ICL3 E 225 - G.H5 H 364 G.H5.09
Hydrophobic TM7 A 296 7x56 G.H5 L 380 G.H5.25
Polar TM6 Q 231 6x30 G.H5 Q 372 G.H5.17
Van-der-waals TM6 Q 231 6x30 G.H5 Q 372 G.H5.17
Polar C-term R 322 - G.H3 N 282 G.H3.14
Hydrophobic C-term R 322 - G.H3 N 282 G.H3.14
Van-der-waals C-term R 322 - G.H3 N 282 G.H3.14
Hydrophobic TM5 L 220 5x65 G.H5 L 375 G.H5.20
Van-der-waals TM5 L 220 5x65 G.H5 L 375 G.H5.20
Hydrophobic ICL3 H 227 - G.h4s6 P 345 G.h4s6.20
Van-der-waals ICL3 H 227 - G.h4s6 P 345 G.h4s6.20
Hydrophobic ICL2 L 129 34x51 G.H5 I 370 G.H5.15
Van-der-waals ICL2 L 129 34x51 G.H5 I 370 G.H5.15
Hydrophobic TM6 Q 231 6x30 G.H5 K 376 G.H5.21
Van-der-waals TM6 Q 231 6x30 G.H5 K 376 G.H5.21
Hydrophobic TM6 R 234 6x33 G.H5 M 379 G.H5.24
Polar C-term S 357 - H.HA K 114 H.HA.28
Van-der-waals C-term S 357 - H.HA K 114 H.HA.28
Hydrophobic C-term F 359 - H.HA R 106 H.HA.20
Van-der-waals C-term F 359 - H.HA R 106 H.HA.20
Hydrophobic TM2 M 62 2x43 G.H5 L 380 G.H5.25
Van-der-waals TM2 M 62 2x43 G.H5 L 380 G.H5.25
Ionic ICL2 R 135 34x57 G.H5 D 377 G.H5.22
Polar ICL2 R 135 34x57 G.H5 D 377 G.H5.22
Van-der-waals ICL2 R 135 34x57 G.H5 D 377 G.H5.22