Gi3 - M5 receptor complex model

GPCR CLASS (FAMILY)

A (Rhodopsin)

SPECIES

Homo sapiens

G PROTEIN FAMILY

Gi/o

DATE

2023-09-15

3D VIEW

NGL is a WebGL based 3D viewer powered by MMTF.


Tertiary structure confidence

(pLDDT score) avg.: 87.3

Very high (>90)
Confident (90-70)
Low (70-50)
Very low (<50)
Disordered

PTM Score: 0.8
iPTM Score: 0.8
PAE mean: 11.2


GPCR - G protein interface interactions



The biflare plot is a modified version of a flareplot, where lines are drawn between an inner concentric circle (Gα residues) and an outer concentric circle (GPCR residues) depicting the interface interactions.
Different interaction types are shown with different line types (aromatic - solid, hydrophobic - dashed, ionic - long dashed, polar - dotted, Van der Waals - dash-dot).
The interactive features include filtering on interaction type, segments, residues and backbone or sidechain interactions.


Interaction cut-off:

Residue numbering:

GPCR-G protein backbone (bb) or sidechain (sc):





Structures

Sequence Color

Gα, GPCR
Interaction cut-off: strict (stick), loose (line)

NGL is a WebGL based 3D viewer powered by MMTF.

Interaction
type		 GPCR
Segment
AA
Number		 Generic
number		 Gα
segment
AA
Number		 Generic
number
Hydrophobic C-term L 531 - G.h2s4 F 215 G.h2s4.01
Van-der-waals C-term L 531 - G.h2s4 F 215 G.h2s4.01
Polar TM6 A 440 6x33 G.H5 L 353 G.H5.25
Polar TM6 T 443 6x36 G.H5 G 352 G.H5.24
Hydrophobic C-term L 531 - G.H2 W 211 G.H2.07
Hydrophobic TM5 R 223 5x68 G.H5 L 348 G.H5.20
Polar ICL3 G 232 - G.S6 T 321 G.S6.03
Hydrophobic ICL2 L 136 34x51 G.S3 L 194 G.S3.01
Van-der-waals ICL2 L 136 34x51 G.S3 L 194 G.S3.01
Hydrophobic TM5 L 230 5x75 G.S6 Y 320 G.S6.02
Van-der-waals TM5 L 230 5x75 G.S6 Y 320 G.S6.02
Hydrophobic TM3 I 132 3x54 G.H5 N 347 G.H5.19
Hydrophobic ICL2 T 137 34x52 G.s2s3 K 192 G.s2s3.01
Polar ICL3 Q 231 - G.S6 I 319 G.S6.01
Hydrophobic ICL3 Q 231 - G.S6 I 319 G.S6.01
Van-der-waals ICL3 Q 231 - G.S6 I 319 G.S6.01
Hydrophobic TM5 L 230 5x75 G.H5 A 338 G.H5.10
Hydrophobic ICL3 V 236 - G.H4 C 305 G.H4.13
Hydrophobic ICL2 L 136 34x51 G.H5 T 340 G.H5.12
Van-der-waals ICL2 L 136 34x51 G.H5 T 340 G.H5.12
Aromatic C-term W 525 - G.H3 W 258 G.H3.17
Hydrophobic C-term W 525 - G.H3 W 258 G.H3.17
Van-der-waals C-term W 525 - G.H3 W 258 G.H3.17
Ionic ICL2 K 141 34x56 G.s2s3 D 193 G.s2s3.02
Polar ICL2 K 141 34x56 G.s2s3 D 193 G.s2s3.02
Van-der-waals ICL2 K 141 34x56 G.s2s3 D 193 G.s2s3.02
Hydrophobic ICL2 P 135 34x50 G.H5 I 343 G.H5.15
Hydrophobic TM5 L 227 5x72 G.h4s6 E 318 G.h4s6.12
Polar TM3 R 128 3x50 G.H5 C 351 G.H5.23
Hydrophobic TM3 R 128 3x50 G.H5 C 351 G.H5.23
Van-der-waals TM3 R 128 3x50 G.H5 C 351 G.H5.23
Polar TM2 N 65 2x39 G.H5 E 350 G.H5.22
Van-der-waals TM2 N 65 2x39 G.H5 E 350 G.H5.22
Hydrophobic TM5 R 223 5x68 G.H5 Y 354 G.H5.26
Polar H8 N 499 8x47 G.H5 E 350 G.H5.22
Van-der-waals H8 N 499 8x47 G.H5 E 350 G.H5.22
Hydrophobic C-term Y 524 - G.H3 K 257 G.H3.16
Van-der-waals C-term Y 524 - G.H3 K 257 G.H3.16
Hydrophobic TM5 T 220 5x65 G.H5 L 348 G.H5.20
Hydrophobic TM6 L 444 6x37 G.H5 L 353 G.H5.25
Polar ICL2 R 139 34x54 G.S1 E 33 G.S1.01
Van-der-waals ICL2 R 139 34x54 G.S1 E 33 G.S1.01
Ionic ICL2 R 142 34x57 G.H5 E 350 G.H5.22
Hydrophobic TM5 L 227 5x72 G.H5 D 341 G.H5.13
Hydrophobic TM5 L 230 5x75 G.H5 F 334 G.H5.06
Van-der-waals TM5 L 230 5x75 G.H5 F 334 G.H5.06
Polar ICL2 R 139 34x54 G.hns1 A 31 G.hns1.02
Hydrophobic ICL2 R 139 34x54 G.hns1 A 31 G.hns1.02
Van-der-waals ICL2 R 139 34x54 G.hns1 A 31 G.hns1.02
Polar ICL2 A 140 34x55 G.hns1 K 32 G.hns1.03
Hydrophobic ICL2 A 140 34x55 G.hns1 K 32 G.hns1.03
Van-der-waals ICL2 A 140 34x55 G.hns1 K 32 G.hns1.03
Hydrophobic TM6 T 443 6x36 G.H5 L 353 G.H5.25
Van-der-waals TM6 T 443 6x36 G.H5 L 353 G.H5.25
Polar ICL2 R 139 34x54 G.hns1 A 30 G.hns1.01
Polar TM6 K 439 6x32 G.H5 Y 354 G.H5.26
Hydrophobic TM6 K 439 6x32 G.H5 Y 354 G.H5.26
Van-der-waals TM6 K 439 6x32 G.H5 Y 354 G.H5.26
Hydrophobic TM7 C 498 7x56 G.H5 G 352 G.H5.24
Van-der-waals TM7 C 498 7x56 G.H5 G 352 G.H5.24
Hydrophobic ICL3 V 236 - G.H4 A 301 G.H4.08
Ionic ICL3 R 429 - G.H4 E 308 G.H4.16
Polar ICL3 R 429 - G.H4 E 308 G.H4.16
Hydrophobic ICL3 R 429 - G.H4 E 308 G.H4.16
Van-der-waals ICL3 R 429 - G.H4 E 308 G.H4.16
Hydrophobic TM3 I 132 3x54 G.H5 L 348 G.H5.20
Van-der-waals TM3 I 132 3x54 G.H5 L 348 G.H5.20
Hydrophobic ICL2 R 139 34x54 G.S3 L 194 G.S3.01
Hydrophobic TM5 R 223 5x68 G.H5 I 344 G.H5.16
Van-der-waals TM5 R 223 5x68 G.H5 I 344 G.H5.16
Hydrophobic TM4 P 144 4x39 G.HN R 24 G.HN.48
Hydrophobic C-term L 531 - G.H2 I 212 G.H2.08
Van-der-waals C-term L 531 - G.H2 I 212 G.H2.08
Hydrophobic ICL2 P 135 34x50 G.H5 I 344 G.H5.16
Van-der-waals ICL2 P 135 34x50 G.H5 I 344 G.H5.16
Hydrophobic C-term S 529 - G.H2 I 212 G.H2.08
Ionic TM5 R 223 5x68 G.h4s6 E 318 G.h4s6.12
Polar TM5 R 223 5x68 G.h4s6 E 318 G.h4s6.12
Hydrophobic TM5 L 230 5x75 G.H5 D 337 G.H5.09
Polar TM6 K 436 6x29 G.H5 Y 354 G.H5.26
Hydrophobic TM6 K 436 6x29 G.H5 Y 354 G.H5.26
Van-der-waals TM6 K 436 6x29 G.H5 Y 354 G.H5.26
Hydrophobic TM5 I 216 5x61 G.H5 L 353 G.H5.25
Hydrophobic ICL2 L 136 34x51 G.S2 F 191 G.S2.08
Hydrophobic TM5 L 227 5x72 G.S6 Y 320 G.S6.02
Van-der-waals TM5 L 227 5x72 G.S6 Y 320 G.S6.02
Hydrophobic TM3 I 132 3x54 G.H5 I 344 G.H5.16
Hydrophobic TM5 R 223 5x68 G.H5 K 345 G.H5.17
Hydrophobic ICL2 L 136 34x51 G.H5 I 343 G.H5.15
Van-der-waals ICL2 L 136 34x51 G.H5 I 343 G.H5.15
Polar ICL3 G 232 - G.H4 Q 304 G.H4.12
Polar TM5 L 230 5x75 G.S6 T 321 G.S6.03
Van-der-waals TM5 L 230 5x75 G.S6 T 321 G.S6.03
Polar ICL3 Q 425 - G.H4 D 309 G.H4.17
Van-der-waals ICL3 Q 425 - G.H4 D 309 G.H4.17
Hydrophobic TM5 L 230 5x75 G.H5 D 341 G.H5.13
Hydrophobic ICL2 R 139 34x54 G.H5 I 343 G.H5.15
Polar ICL3 Q 425 - G.H4 C 305 G.H4.13
Hydrophobic ICL3 Q 425 - G.H4 C 305 G.H4.13
Van-der-waals ICL3 Q 425 - G.H4 C 305 G.H4.13
Hydrophobic ICL2 L 136 34x51 G.s2s3 K 192 G.s2s3.01
Van-der-waals ICL2 L 136 34x51 G.s2s3 K 192 G.s2s3.01
Hydrophobic ICL2 P 135 34x50 G.H5 T 340 G.H5.12
Van-der-waals ICL2 P 135 34x50 G.H5 T 340 G.H5.12
Ionic H8 R 500 8x48 G.h3s5 E 261 G.h3s5.02
Hydrophobic ICL2 A 140 34x55 G.s2s3 D 193 G.s2s3.02
Van-der-waals ICL2 A 140 34x55 G.s2s3 D 193 G.s2s3.02
Polar ICL3 Q 231 - G.H4 Q 304 G.H4.12
Van-der-waals ICL3 Q 231 - G.H4 Q 304 G.H4.12
Hydrophobic C-term N 528 - G.H3 W 258 G.H3.17
Hydrophobic TM4 P 144 4x39 G.HN E 28 G.HN.52
Van-der-waals TM4 P 144 4x39 G.HN E 28 G.HN.52
Ionic TM5 R 223 5x68 G.H5 D 341 G.H5.13
Polar TM5 R 223 5x68 G.H5 D 341 G.H5.13
Hydrophobic TM5 R 223 5x68 G.H5 D 341 G.H5.13
Van-der-waals TM5 R 223 5x68 G.H5 D 341 G.H5.13
Ionic TM6 K 436 6x29 G.h4s6 E 318 G.h4s6.12
Polar TM6 K 436 6x29 G.h4s6 E 318 G.h4s6.12
Van-der-waals TM6 K 436 6x29 G.h4s6 E 318 G.h4s6.12
Polar ICL3 S 235 - G.H4 Q 304 G.H4.12
Van-der-waals ICL3 Q 425 - G.H4 E 308 G.H4.16
Polar TM3 S 131 3x53 G.H5 N 347 G.H5.19
Hydrophobic TM3 S 131 3x53 G.H5 N 347 G.H5.19
Van-der-waals TM3 S 131 3x53 G.H5 N 347 G.H5.19
Hydrophobic ICL2 A 140 34x55 G.S3 L 194 G.S3.01
Hydrophobic ICL3 Q 231 - G.S6 T 321 G.S6.03